Induction of resistance vessel dilation by ginger root juice, possibly through extracellular signal-regulated kinase 1/2 and endothelial nitric oxide synthase activation in endothelial cells
Authors: Bird, Elizabeth; Shah, Dinesh M.
To investigate the effects of ginger root juice on contractibility of resistance blood vessels from mice and on activation of extracellular signal-regulated kinase 1/2 (ERK1/2) and endothelial nitric oxide synthase (eNOS) in human endothelial cells.
Methods: Juice was prepared from semi-dry ginger roots. Mesenteric artery rings were dissected from healthy adult C57BL/J6 mice. Primary human umbilical vein endothelial cells (HUVECs) were isolated from umbilical cords of normal full-term babies. The contractibility of the dissected vessel rings in the presence or absence of ginger root juice at 0.1% (v/v) after potassium (100 mM KCL) stimulation was measured by wire myography. The phosphorylation levels of ERK1/2 and eNOS in the presence of ginger root juice in the culture medium at 0, 0.025%, 0.05%, 0.1%, and 0.2% (v/v) in HUVECs were assessed by western blotting analysis.
Results: An immediate sharp increase in the contractile activity was observed in mesenteric artery rings in response to KCL stimulation. Ginger root juice effectively attenuated the KCL-mediated vessel contraction. Moreover, ginger root juice significantly increased phosphorylation of ERK1/2 and eNOS in a dose-dependent manner.Conclusions: Ginger root juice is capable of relaxing resistance blood vessels. Activation of ERK1/2 and eNOS through phosphorylation in endothelial cells may be a mechanism underlying the vasodilator activity of ginger root.
Document Type: Research Article