KCNQ1 Gene Polymorphism and Type 2 Diabetes Mellitus

Correlation between KCNQ1 gene polymorphism and type 2 diabetes mellitus in Huaihai region of China

Authors: Gao, Xing ; LV, Dongmei; Wang, Yan; Wang, Tao; Li, Wei; Zhang, Yanbo ; Wei, Yaqin; Zhang, Jiali ; Lu, Qian; Yin, Xiaoxing

Objective: This article aims to discuss the distribution of KCNQ1 gene polymorphism in the Chinese Han population in the Huaihai region of China and the correlation between KCNQ1 gene polymorphism and incidence of type 2 diabetes (T2DM).
Methods: From December 2010 to July 2011, 20 0 T2DM inpatients and outpatients in the Endocrinology Department of the Affiliated Hospital of Xuzhou Medical College were selected as the case group and, 200 healthy people identifi ed by the health examination center in the same region were selected as the control group. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test was used to examine the gene polymorphism of the two groups.
Results: (1) Analysis on the control group showed that at the KCNQ1 rC237892 locus, the genotype frequencies of CC, CT and TT were 36.0% (72/200), 51.0% (102/200) and 13.0% (26/200) respectively, and the allelic frequencies of C and T were 61.5% (246/400) and 38.5% (154/400) respectively. Analysis on the case group showed the genotype frequencies of CC, CT and TT were 47.5% (95/200), 44.0% (88/200) and 8.5% (17/200) respectively, and the allelic frequencies of C and T were 69.5% (278/400) and 30.5% (122/400) respectively. Comparison between the genotype distributions and allelic frequencies of the two tested groups at KCNQ1 rC237892 locus showed differences with statistical signifi cance (P<0.05). (2) Comparison between the genotype distributions and allelic C and A frequencies of the control group and the case group showed differences with no statistical signifi cance (P>0.05).
Conclusion: Polymorphism at KCNQ1 rs2237892 locus may be correlated to the incidence of T2DM in the Chinese Han population in Huaihai region of China; polymorphism at rsl51290 locus may be irrelevant to the incidence of T2DM in the Chinese Han population in Huaihai region of China.